Quick start


To profile a metagenomic or metatranscriptomic sample, install the tool and follow the instructions in the tutorial. If you use this tool in published work, please cite us.

Summary


Phylogenetic markers are genes that can be used to reconstruct the evolutionary history of organisms and to profile the taxonomic composition of environmental samples. Efforts to find a good set of protein-coding phylogenetic marker genes led to the identification of 40 universal marker genes (MGs) [1,2]. These 40 MGs occur in single copy in the vast majority of known organisms and they have been used to delineate prokaryotic organisms at the species level [3].

We developed the mOTU profiler as a successor of the original version described in [4]. It uses 10 of the 40 MGs to taxonomically profile shotgun metagenomes, to quantify metabolically active members in metatranscriptomics and to quantify differences between strain populations using single nucleotide variation (SNV) profiles. We extracted the MGs from ~25,000 prokaryotic reference genomes and more than 3,100 publicly available metagenomes (from major human body sites, gut microbiome samples from disease association studies, and ocean water samples). Clustering of MGs led to the generation of a database of MG-based operational taxonomic units (mOTUs) containing 2,494 metagenomic mOTUs (meta-mOTUs) and 5,232 reference mOTUs (ref-mOTUs). Alignments against this database are then used to taxonomically classify reads, to identify metabolically active members and to profile sub-species level SNVs.